Srinivasan Kokatam1, Kanchan Tiwari1, Jenny Schroeder2, Andrea Toell2, Lubna Hussain3, Preeti Kapoor1.

1. Lonza India Pvt Ltd, Hyderabad, India; 2. Lonza Cologne GmbH, Cologne, Germany; 3. Lonza Walkersville, Inc., Walkersville, MD, U.S.A.

 

 

 

1. 使用pmaxGFP質粒進行進行預實驗，優化HUVEC細胞核轉條件；

2. 檢測核轉條件對HUVEC細胞的Tube formation是否有影響；

3. 轉染siRNA，確定對細胞活力影響最小的最佳濃度，確定檢測蛋白表達降低的最佳時間點；

4. 評價siRNA對HUVEC細胞Tube Formation的影響。

 

圖1. HUVEC細胞轉染GFP質粒，轉染效率約為82%，對細胞形態無明顯影響：

圖2. HUVEC細胞進行核轉后，對Tube formation無明顯影響：

圖3. Anti-VEGFR2 siRNA轉染后的細胞對VEGF無反應，證明siRNA發揮作用。

 

 

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6. Kapoor, R., Reddy, V. and Kapoor, P. (2014) Tube Formation Assay with Primary Human Umbilical Vein Endothelial Cells. Resource Notes, 2014: 9-12.