通過可控制的微波加熱進行快速固相多肽合成
Rapid solid-phase peptide synthesis using thermal and controlled microwave irradiation
Abstract: A rapid and efficient microwave-assisted solid-phase synthesis method is described for the preparation of thenonapeptideWDTVRISFK, using conventional Fmoc/But orthogonal protection strategy. The synthesis protocol is based on theuse of cycles of pulsed microwave irradiation with intermittent cooling of the reaction during the removal of the Fmoc protectinggroup and during the coupling. The desired nonapeptide was obtained in highest yield and purity by employing MicroKantechnology. The chemical reactions were carried out in a single-mode microwave reactor, equipped with a fiber-optic probe tomonitor the reaction temperature continuously.
通過可控制的微波加熱進行快速固相多肽合成
摘要：本文描述一種快速有效的微波輔助固相合成方法，用以合成序列為WDTVRISFK的短肽，使用傳統(tǒng)的Fmoc（9-芴氧羰基）/tBu（叔丁基）保護基策略。該合成方法是基于反應(yīng)中的周期性脈沖微波輻射和間歇性冷卻技術(shù)，在Fmoc保護基的脫除及偶聯(lián)反應(yīng)中均應(yīng)用此技術(shù)。在應(yīng)用微反應(yīng)器技術(shù)后得到了高純度和高收率的目標多肽。該反應(yīng)在一個CEM單模微波反應(yīng)器中進行，并且使用光纖進行連續(xù)測溫。
原文出處：
Rapid solid-phase peptide synthesis using thermal andcontrolled microwave irradiation
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