這是第一次報告多電極記錄運動神經元網絡。從E15大鼠脊髓腹角分離出來的神經細胞放在Med-64探針里培養。大多數培養的神經元具有神經絲、膽堿乙酰轉移酶和Hb9 等運動神經元的特點。運動神經元網絡的活動特點是具有單個細胞尖峰，并且是自發的。尖峰和網絡突發是由γ-氨基丁酸拮抗劑 (GABA)和乙酰膽堿刺激引起的，也能被GABA和谷氨酸受體拮抗劑本身所抑制。培養的胚胎運動神經元網絡是一個研究運動神經元發展、運動神經疾病生理及病理機制的理想模型系統。

Neuronal and network activity in networks of cultured spinal motor neurons.

Zhang HM, Robinson N, Gómez-Curet I, Wang W, Harrington MA.

Department of Biological Sciences, Delaware State University, Dover, DE 19901, USA.

This is the first report of multielectrode recordings from networks of cultured motor neurons. Neurons isolated from the ventral horns of spinal cords of E15 rats were cultured on MED64 probes. The majority of the neurons in the cultures are positive for neurofilament, choline acetyltransferase, and Hb9, characteristics of motor neurons. The activity of the motor neuron network is characterized by spiking of individual cells as well as spontaneous, synchronized bursts involving all active electrodes. Both spiking and network bursts are stimulated by GABA antagonists and acetylcholine, and are inhibited by GABA itself and glutamate antagonists. Networks of cultured embryonic motor neurons make a good model system for studying motor neuron development and physiology as well as the pathophysiology of motor neuron disease.

文章來源：http://www.ncbi.nlm.nih.gov/pubmed/19436229?ordinalpos=1&itool=EnvportPanel.Pubmed_RVDocSum