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2120 次 微環境多靶標蛋白分析系統升級前后功能對比 2022-10-21
深入分析組織微環境對于理解疾病生物學及其進展至關重要。過去幾年，芯片流式技術ChipCytometry產品在組織免疫分析中展現出的巨大應用價值，已經得到了世界各地用戶的廣泛認可和肯定，這種先進的
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16147 次 CellSens standard軟件主要功能操作說明 2018-5-22
一、明場實時圖像調節及拍照保存1.打開cellSens軟件后，首先在攝像控制工具窗口中切換相機芯片為“彩色模式”，此時相機自動切換為彩色芯片進行明場圖像采圖；接下來點擊“實時觀
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2627 次 脾臟、胸腺和淋巴結中單個核細胞分離和培養 2017-3-16
基本方案從小鼠脾臟、胸腺和淋巴結制備單個核細胞分離和培養。實驗材料1.RPMI-5或DMEM-5完全培養基。2.新鮮分離的小鼠器官：≤6周齡的小鼠胸腺；6周至6個月齡的小鼠脾臟和淋巴結。3.60mm&time
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2378 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr
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3246 次 Culture of human venous smooth muscle cells 2012-5-23
Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f
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3072 次 Isolation of human prostatic smooth muscle cells 2012-5-8
Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of
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2823 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
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1783 次 Isolation of papillary cells 2012-3-26
Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept
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5628 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc
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3226 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli
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3902 次 Isolation of human primary ovarian surface epithelial cells 2011-12-12
Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut
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1627 次 Isolation of human prostatic epithelial cells 2011-11-25
Isolation of human prostatic epithelial cells1.A small piece of tissue from each specimen was removed and minced. 2.The tissue was digested with collagenase overnight. 3.To remove
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3306 次 Isolation of stromal vascular cells from human adipose tissue 2011-9-30
Isolation of stromal vascular cells from human adipose tissue Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal ste
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3381 次 Isolation and cultivation of endothelial progenitor cells (EPCs) 2011-9-30
Isolation and cultivation of endothelial progenitor cells (EPCs) Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue rege
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2328 次 Isolation of rodent pancreatic β cells 2011-8-1
Isolation of rodent pancreatic β cells 1.Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.Rat islets were isolated from mal
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2365 次 Culture of human renal proximal tubule cells and renal cortical fibroblasts 2011-7-11
Culture of human renal proximal tubule cells and renal cortical fibroblasts 1.The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibrobla
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2115 次 Isolation of human colonic epithelial cells (EC) 2011-6-21
Isolation of human colonic epithelial cells (EC)1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were
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2451 次 Isolation and Culture of Human Liver Stem Cells 2011-4-1
Isolation and Culture of Human Liver Stem CellsMaterials and Methods1.Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy li
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2186 次 FITC標記抗體-Marsshall氏法 2010-10-13
FITC標記抗體-Marsshall氏法材料：抗體球蛋白溶液、0.5mol/L pH9.0碳酸鹽緩沖液、無菌生理鹽水、異硫氰酸熒光素、1%硫柳汞水溶液、三角燒瓶（25—50ml）、冰及冰槽（或1000ml燒杯）、電磁攪拌器
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3043 次 原代細胞骨架的染色方法 2010-10-11
原代細胞骨架的染色方法微絲的顯示方法步驟：1. 用PBS液漂洗蓋片培養的原代細胞3次，每次30s；2. 用2%的甲醛/PBS液固定原代細胞3min；3. 用0.5%的三硝基甲苯/PBS處理3次，每次10min；4. PBS漂洗
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